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KMID : 0043320010240040349
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Archives of Pharmacal Research
2001 Volume.24 No. 4 p.349 ~ p.354
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Inhibition of Invasion and Induction of Apoptosis by Curcumin in H-ras-Transformed MCF10A Human Breast Epithelial Cells
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Kim MS
Kang HJ/Moon AR
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Abstract
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Curcumin, a dietary pigment in turmeric, posseses anti-carcinogenic and anti- metastatic properties. The present study was conducted to study in vitro chemopreventive effects of curcumin in transformed breast cells. Here, we show that curcumin inhibits H-ras-induced invasive phenotype in MCF10A human breast epithelial cells (H-ras MCF10A) and down-regulates matrix metalloproteinase (MMP)-2 dose- dependently. Curcumin exerted cytotoxic effect on H-ras MCF10A cells in a concentration-dependent manner. Curcumin-induced cell death was mainly due to apoptosis in which a prominent downregulation of Bcl-2 and upregulation of Bax were involved. We also suggest a possible involvement of caspase-3 incurcumin-induced apoptosis. Curcumin treatment resulted in the production of reactive oxygen species (ROS) in H-ras MCF10A cells. Apoptotic event by curcumin was significantly intribited by pretreatment of an antioxidant N-acetyl-L-cysteine (NAC), suggesting redox signaling as a mechanism responsible for curcumin-induced apoptosis in H-ras MCF10A cells. Taken togethers our results demonstrate that curcumin inhibits invasion and induces apoptosis, proving the chemopreventive potential of curcumin.
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